Imaging Artificial Membranes Using High-Speed Atomic Force Microscopy

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Nasrallah, Hussein | Vial, Anthony | Pocholle, Nicolas | Soulier, Jérémy | Costa, Luca | Godefroy, Cédric | Bourillot, Eric | Lesniewska, Eric | Milhiet, Pierre-Emmanuel

Edité par CCSD ; Humana Press/Springer Imprint -

International audience. Supported lipid bilayers represent a very attractive way to mimic biological membranes, especially to investigate molecular mechanisms associated with the lateral segregation of membrane components. Observation of these model membranes with high-speed atomic force microscopy (HS-AFM) allows the capture of both topography and dynamics of membrane components, with a spatial resolution in the nanometer range and image capture time of less than 1 s. In this context, we have developed new protocols adapted for HS-AFM to form supported lipid bilayers on small mica disks using the vesicle fusion or Langmuir-Blodgett methods. In this chapter we describe in detail the protocols to fabricate supported artificial bilayers as well as the main guidelines for HS-AFM imaging of such samples.

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