Histidine-Rich Cationic Cell-Penetrating Peptides for Plasmid DNA and siRNA Delivery

0 avis

Histidine-Rich Cationic Cell-Penetrating Peptides for Plasmid DNA and siRNA Delivery

Archive ouverte

Kichler, Antoine | Mason, A-James | Marquette, Arnaud | Bechinger, Burkhard

International audience. Amphipathic, pH-responsive, membrane-active peptides such as LAH4 and derivatives thereof have the ability to effectively deliver genes and small interfering RNA (siRNA) into mammalian cells. Their ability to bind and protect nucleic acids and then disrupt membranes when activated at low pH enables them to harness the endocytic machinery to deliver cargo efficiently and with low associated toxicity. This chapter describes protocols for the chemical synthesis of transfection peptides of the LAH4 family, complex formation with nucleic acids, and their use for the in vitro delivery of either plasmid DNA or siRNA into mammalian cell lines.

Langue: en

Indexation

Consulter en ligne

Consulter le document

Suggestions

Du même auteur

Peptides derived from the C-terminal domain of HIV-1 Viral Protein R in lipid bilayers: Structure, membrane positioning and gene delivery

Archive ouverte | Marquette, Arnaud | CCSD

International audience. Viral protein R (Vpr) is a small accessory protein of 96 amino acids that is present in Human and simian immunodeficiency viruses. Among the very different properties that Vpr possesses we ca...

Membrane Interaction of Chrysophsin-1, a Histidine-Rich Antimicrobial Peptide from Red Sea Bream †

Archive ouverte | Mason, A. James | CCSD

International audience. Chrysophsin-1 is an amphipathic alpha-helical antimicrobial peptide produced in the gill cells of red sea bream. The peptide has broad range activity against both Gram-positive and Gram-negat...

Histidine-Rich Designer Peptides with pH-Dependent Membrane Topology, Antimicrobial, Nucleic Acid Transfection and Viral Transduction Capabilities

Archive ouverte | Aisenbrey, Christopher | CCSD

Chargement des enrichissements...