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Long-Term In vitro Cultivation of Plasmodium falciparum in a Novel Cell Culture Device

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Géry, Antoine | Basco, Leonardo | Heutte, Natacha | Guillamin, Marilyne | N’guyen, Ho-Mai-Thy | Richard, Estelle | Garon, David | Eldin de Pécoulas, Philippe

Edité par CCSD ; American Society of Tropical Medicine and Hygiene -

International audience. The standard in vitro cultivation procedure for Plasmodium falciparum requires gas exchange and a microaerophilic atmosphere. A novel system using a commercially available cell culture device (Petaka G3™; Celartia Ltd., Powell, OH) was assessed for long-term cultivation of a P. falciparum reference laboratory clone in normal air. Parasite growth during 30 days was similar, or better, in Petaka G3 than that in the standard cultivation method with gas exchange in a CO2 incubator. The successful cultivation of P. falciparum in the Petaka G3 device suggests that low O2 content available in hemoglobin and dissolved gas in the blood is sufficient for long-term cultivation. This finding may open the way to novel methods to cultivate and adapt P. falciparum field isolates to in vitro conditions with more ease.

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Long-Term In vitro Cultivation of Plasmodium falciparum in a Novel Cell Culture Device

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Long-Term In vitro Cultivation of Plasmodium falciparum in a Novel Cell Culture Device. Mme/Mr HAL, Bonjour, Je ne trouve pas votre adresse mail et vous m’adressez vos mails à partir d’une adresse « no reply ».Cet article est disponible gratuitement via Pubmed. Il ne faut pas aller au site web du journal Am J Trop Med Hyg (DOI: 10.4269/ajtmh.18-0527), réservé aux membres de la société américaine de médecine tropicale.Je vous remercie d’y accéder en allant à :https://pubmed.ncbi.nlm.nih.gov/30693863ouhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447126/cordialement,Leonardo Basco

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