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Multiparametric radiobiological assays show that variation of X-ray energy strongly impacts relative biological effectiveness: comparison between 220 kV and 4 MV

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Paget, Vincent | Ben Kacem, Mariam | dos Santos, Morgane | Benadjaoud, Mohamed Amine | Soysouvanh, Frédéric | Buard, Valerie | Tarlet, Georges | Vaurijoux, Aurelie | Gruel, Gaetan | Francois, Agnes | Guipaud, Olivier | Milliat, Fabien

Edité par CCSD ; Nature Publishing Group -

International audience. Based on classic clonogenic assay, it is accepted by the scientific community that, whatever the energy, the relative biological effectiveness of X-rays is equal to 1. However, although X-ray beams are widely used in diagnosis, interventional medicine and radiotherapy, comparisons of their energies are scarce. We therefore assessed in vitro the effects of low- and high-energy X-rays using Human umbilical vein endothelial cells (HUVECs) by performing clonogenic assay, measuring viability/mortality, counting γ-H2AX foci, studying cell proliferation and cellular senescence by flow cytometry and by performing gene analysis on custom arrays. Taken together, excepted for γ-H2AX foci counts, these experiments systematically show more adverse effects of high energy X-rays, while the relative biological effectiveness of photons is around 1, whatever the quality of the X-ray beam. These results strongly suggest that multiparametric analysis should be considered in support of clonogenic assay.

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