Afficher la couverture 'Molecular structure of promoter-bound yeast TFIID | Kolesnikova, Olga' en grand format
/5

0 avis

Molecular structure of promoter-bound yeast TFIID

Archive ouverte

Kolesnikova, Olga | Ben-Shem, Adam | Luo, Jie | Ranish, Jeff | Schultz, Patrick | Papai, Gabor

Edité par CCSD ; Nature Publishing Group -

International audience. Transcription preinitiation complex assembly on the promoters of protein encoding genes is nucleated in vivo by TFIID composed of the TATA-box Binding Protein (TBP) and 13 TBPassociate factors (Tafs) providing regulatory and chromatin binding functions. Here we present the cryo-electron microscopy structure of promoter-bound yeast TFIID at a resolution better than 5 Å, except for a flexible domain. We position the crystal structures of several subunits and, in combination with cross-linking studies, describe the quaternary organization of TFIID. The compact tri lobed architecture is stabilized by a topologically closed Taf5-Taf6 tetramer. We confirm the unique subunit stoichiometry prevailing in TFIID and uncover a hexameric arrangement of Tafs containing a histone fold domain in the Twin lobe.

Suggestions

Du même auteur

Structure of SAGA and mechanism of TBP deposition on gene promoters

Archive ouverte | Papai, Gabor | CCSD

International audience. SAGA (Spt–Ada–Gcn5–acetyltransferase) is a 19-subunit complex that stimulates transcription via two chromatin-modifying enzymatic modules and by delivering the TATA box binding protein (TBP) ...

Structure of the human TIP60-C histone exchange and acetyltransferase complex

Archive ouverte | Li, Changqing | CCSD

International audience. Chromatin structure is a key regulator of DNA transcription, replication and repair1. In humans, the TIP60–EP400 complex (TIP60-C) is a 20-subunit assembly that affects chromatin structure th...

Architecture of the multi‐functional SAGA complex and the molecular mechanism of holding TBP. architecture du complexe multifonctionnel SAGA et mécanisme moléculaire de son interaction avec TBP

Archive ouverte | Ben-Shem, Adam | CCSD

International audience. In eukaryotes, transcription of protein encoding genes is initiated by the controlled deposition of the TATA-box binding protein TBP onto gene promoters, followed by the ordered assembly of a...

Chargement des enrichissements...