How einkorn (Triticum monoccocum) deals with variable nitrogen (N) and sulphur (S) supply during grain development? Exploration through proteome investigation

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Bancel, Emmanuelle | Bonnot, Titouan | Alvarez, David | Davanture, Marlène | Zivy, Michel | Martre, Pierre | Ravel, Catherine

Edité par CCSD -

Book of abstracts, ISBN: 978-80-970662-3-9, EAN: 9788097066239 Poster p. 52
Book of abstracts, ISBN: 978-80-970662-3-9, EAN: 9788097066239Poster p. 52. The concentration and composition of storage proteins in mature wheat grain and consequently its quality are strongly affected by nitrogen (N) and sulphur (S) supply1. Einkorn (Triticum monoccocum), a wheat diploid species was used as a model of bread wheat (T. aestivum). Einkorn plants were grown in a greenhouse under controlled conditions with different N and S amounts. Grains were harvested at different stages from 300 to 600°Cd corresponding to the filling phase of grain development. Proteomics approach by gel-free mass spectrometry analysis was used to study effects of N and S nutrition on metabolic proteins (albumins-globulins).The accumulation of storage proteins was affected by N and S supply, with significant changes in the grain composition at maturity. In particular, the accumulation of S-rich and S-poor storage proteins were differentially impacted by S supply. Some metabolic proteins (155 albumins-globulins) were also differentially accumulated in response to the nutrition. A number of proteins involved in key biological functions were either up- or down-regulated. We focus on the data set obtained in response to S supply in order to highlight some proteins and underline mechanisms occurring in response to nutrition in wheat grain.

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