Syracuse

Biologically active lipid A antagonist embedded in a multilayered polyelectrolyte architecture

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Gangloff, Sophie, C. | Ladam, Guy | Fukase, Kochi | Brandenburg, Klaus | Guenounou, Moncef | Schaaf, Pierre | Voegel, Jean-Claude | Jessel, Nadia | Dupray, Valérie

Edité par CCSD ; Elsevier -

International audience. Recently [Jessel N, Schwinte P, Donohue R, Lavalle P, Boulmedais F, Darcy R, et al. Pyridylamino-b-cyclodextrin as a molecular chaperone for lipopolysaccharide embedded in a multilayered polyelectrolyte architecture. Adv Funct Mater 2004;14:963-9], we demonstrated the biological activity of a lipopolysaccharide from Escherichia coli incorporated into layer-by-layer films made of poly (L-lysine) and poly (L-glutamic acid) and containing a polycationic b-cyclodextrin (CD) with chaperone properties. Here we develop innovative architectures containing a complex made of a charged b-cyclodextrin and a lipid A antagonist (LAA) as potential systems for local endotoxin antagonistic activity. We examine the biological activity of these architectures. The CD-LAA complex adsorbed on top, or embedded into the polyelectrolyte films keeps its LPS antagonistic activity on both murine and human macrophages for at least 24 h. r

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