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How Detergent Impacts Membrane Proteins: Atomic-Level Views of Mitochondrial Carriers in Dodecylphosphocholine

Archive ouverte | Kurauskas, Vilius | CCSD

International audience. Characterizing the structure of membrane proteins (MPs) generally requires extraction from their native environment, most commonly with detergents. Yet, the physicochemical properties of dete...

Cross-Correlated Relaxation of Dipolar Coupling and Chemical-Shift Anisotropy in Magic-Angle Spinning R 1ρ NMR Measurements: Application to Protein Backbone Dynamics Measurements

Archive ouverte | Kurauskas, Vilius | CCSD

International audience. Transverse relaxation rate measurements in MAS solid-state NMR provide information about molecular motions occurring on nanoseconds-to-milliseconds (ns-ms) time scales. The measurement of het...

Protein conformational dynamics studied by (15)N and (1)H R1ρ relaxation dispersion: Application to wild-type and G53A ubiquitin crystals.

Archive ouverte | Gauto, Diego | CCSD

International audience. Solid-state NMR spectroscopy can provide site-resolved information about protein dynamics over many time scales. Here we combine protein deuteration, fast magic-angle spinning (~45-60kHz) and...

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