Developmental control of plant Rho GTPase nano-organization by the lipid phosphatidylserine

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Platre, Matthieu Pierre | Bayle, Vincent | Armengot, Laia | Bareille, Joseph | Marquès-Bueno, Maria del Mar | Creff, Audrey | Maneta-Peyret, Lilly | Fiche, Jean-Bernard | Nollmann, Marcelo | Miege, Christine | Moreau, Patrick | Martinière, Alexandre | Jaillais, Yvon

Edité par CCSD ; American Association for the Advancement of Science (AAAS) -

International audience. Rho GTPases are master regulators of cell signaling, but how they are regulated depending on the cellular context is unclear. Here, we show that the phospholipid phosphatidylserine acts as a 25 developmentally-controlled lipid rheostat that tunes Rho GTPase signaling in Arabidopsis. Live super-resolution single molecule imaging revealed that RHO-OF-PLANT6 (ROP6) is stabilized by phosphatidylserine into plasma membrane nanodomains, which is required for auxin signaling. Furthermore, we uncovered that the plasma membrane phosphatidylserine content varies during plant root development and that the level of phosphatidylserine modulates the quantity of ROP6 30 nanoclusters induced by auxin and hence downstream signaling, including regulation of endocytosis and gravitropism. Our work reveals that variations in phosphatidylserine levels are a physiological process that may be leveraged to regulate small GTPase signaling during development. One Sentence Summary: 35 Phosphatidylserine acts as a developmentally-controlled lipid rheostat that regulates auxin sensitivity and plant development.

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