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Flexible-to-rigid transition is central for substrate transport in the ABC transporter BmrA from Bacillus subtilis

Archive ouverte | Lacabanne, Denis | CCSD

International audience. ATP-binding-cassette (ABC) transporters are molecular pumps that translocate molecules across the cell membrane by switching between inward-facing and outward-facing states. To obtain a detai...

Cell-free expression, purification, and membrane reconstitution for NMR studies of the nonstructural protein 4B from hepatitis C virus

Archive ouverte | Fogeron, Marie-Laure | CCSD

International audience. We describe the expression of the hepatitis C virus nonstructural protein 4B (NS4B), which is an integral membrane protein, in a wheat germ cell-free system, the subsequent purification and c...

Efficient and stable reconstitution of the ABC transporter BmrA for solid-state NMR studies

Archive ouverte | Kunert, Britta | CCSD

Affiliations ECOFECT. International audience. We present solid-state NMR sample preparation and first 2D spectra of the Bacillus subtilis ATP-binding cassette (ABC) transporter BmrA, a membrane protein involved in m...

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