Afficher la couverture 'The human macrophage galactose‐type lectin, MGL, recognizes the outer core of E. coli lipooligosaccharide | Maalej, Meriem' en grand format
/5

0 avis

The human macrophage galactose‐type lectin, MGL, recognizes the outer core of E. coli lipooligosaccharide

Archive ouverte

Maalej, Meriem | Forgione, Rosa, Ester | Marchetti, Roberta | Bulteau, Francois | Thépaut, Michel | Lanzetta, Rosa | Laguri, Cédric | Simorre, Jean-Pierre | Fieschi, Franck | Molinaro, Antonio | Silipo, Alba

Edité par CCSD ; Wiley-VCH Verlag -

International audience. Carbohydrate-lectin interactions intervene in and mediate most biological processes, including a crucial modulation of immune responses to pathogens. Despite the growing interest in investigating the association between host receptor lectins and exogenous glycan ligands, the molecular mechanisms underlying bacterial recognition by human lectins are still not fully understood. Here we describe a novel molecular interaction between the human macrophage galactose-type lectin (MGL) and the lipooligosaccharide (LOS) of E. coli strain R1. Saturation Transfer Difference (STD) NMR analysis, supported by computational studies, demonstrated that MGL bound to the purified deacylated LOSR1 mainly through the recognition of its outer core and established crucial interactions with the terminal Galα(1,2)Gal epitope. These results assess the ability of MGL to recognize glycan moieties exposed on Gram-negative bacterial surfaces.

Suggestions

Du même auteur

The unique 3D arrangement of macrophage galactose lectin enables Escherichia coli lipopolysaccharide recognition through two distinct interfaces

Archive ouverte | Abbas, Massilia | CCSD

International audience. Lipopolysaccharides are a hallmark of gram-negative bacteria, and their presence at the cell surface is key for bacterial integrity. As surface-exposed components, they are recognized by immu...

Solid State NMR Studies of Intact Lipopolysaccharide Endotoxin

Archive ouverte | Laguri, Cédric | CCSD

International audience. Lipopolysaccharides (LPS) are complex glycolipids forming the outside layer of Gram-negative bacteria. Their hydrophobic and heterogeneous nature greatly hampers their structural study in an ...

Atomic-Level Dissection of DC-SIGN Recognition of Bacteroides vulgatus LPS Epitopes

Archive ouverte | Nieto-Fabregat, Ferran | CCSD

International audience. The evaluation of Bacteroides vulgatus mpk (BVMPK) lipopolysaccharide (LPS) recognition by DC-SIGN, a key lectin in mediating immune homeostasis, has been here performed. A fine chemical diss...

Chargement des enrichissements...