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Sequence Analysis of env C2/V3, gag p17/p24, and pol Protease Regions of 25 HIV Type 1 Isolates from Ho Chi Minh City, Vietnam

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Caumont, Anne | Lan, Nguyen Thi Hoang | Uyen, Nguyen Thi Vy | Hung, Pham Van | Schvoerer, Evelyne | Urriza, Maria-Soledad Goni | Roques, Pierre | Schrive, Marie-Helene | Lien, Truong Thi Xuan | Lafon, Marie-Edith | Dormont, Dominique | Barre-Sinoussi, Francoise | Fleury, Herve J.A.

Edité par CCSD ; Mary Ann Liebert -

International audience. Env C2/V3, gag p17/p24, pol protease, and RT regions of HIV-1 isolates recently obtained from 25 HIV-1 seropositive individuals from Ho Chi Minh City (Vietnam) were studied, and genes subtypes were determined by DNA sequence analyses. Twenty-three isolates out of 25 were identified as belonging to subtype E, now recognized as circulating recombinant form 1 (CRF01_AE). The motif at the top of the V3 loop (generally GPGQ) was then preceded by an isoleucine or a methionine (M) residue; the M residue might be a local signature of Vietnamese E isolates compared to Thai E viruses. Two isolates (8%) were shown to be intersubtype recombinants: one E/B and one CRF02_AG(IBNG)/D. The polymorphism of pol protease was considered only for CRF01_AE isolates and is clearly different from that recorded for B viruses with substitutions at positions 13, 35, 36, 41, 69, and 89.

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