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Flow cytometric enumeration of bacterial in the coral surface mucus layer

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Bettarel, Yvan | Mai, Thanh Chi | Got, Patrice | Adingra, Antoinette | Kouadio-Ngbesso, Nadège | van Ngoc, Bui | Bouvier, Thierry

Edité par CCSD ; Elsevier -

International audience. The direct counts of bacteria inhabiting coral mucus were performed by flow cytometry testing four fluorescent dyes (SYBR®Green I, HCS, TOPRO®3, SYTO®62) with three different scleractinian species. Results obtained with SYTO62 were the most reliable based on the comparison with standardized epifluorescence counts and the resolution of cytograms.

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