Imaging Plastids in 2D and 3D: Confocal and Electron Microscopy.

0 avis

Imaging Plastids in 2D and 3D: Confocal and Electron Microscopy.

Archive ouverte

Edité par CCSD ; Springer -

International audience. Internal chloroplast structures present complex and various characteristics, which are still largely undetermined due to insufficient imaging investigation. Information on chloroplast morphology has traditionally been collected using light microscopy (LM), confocal laser scanning microscopy (CLSM), transmission electron microscopy (TEM), and scanning electron microscopy (SEM) techniques. However, recent technological progresses in the field of microscopy have made it possible to visualize the internal structure of chloroplast in far greater detail and in 3D. Here we recapitulate protocols to visualize chloroplasts from Arabidopsis leaves and Phaeodactylum tricornutum cells with confocal and transmission electron microscopy together with a new technique using a focused ion beam-scanning electron microscope (FIB-SEM) allowing for 3D imaging.

Langue: en

ISBN: : 978-1-4939-8654-5; 978-1-4939-8653-8

Indexation

Consulter en ligne

Consulter le document

Suggestions

Du même auteur

Plastid thylakoid architecture optimizes photosynthesis in diatoms.

Archive ouverte | Flori, Serena | CCSD

International audience. Photosynthesis is a unique process that allows independent colonization of the land by plants and of the oceans by phytoplankton. Although the photosynthesis process is well understood in pla...

Morphological bases of phytoplankton energy management and physiological responses unveiled by 3D subcellular imaging

Archive ouverte | Uwizeye, Clarisse | CCSD

International audience. Eukaryotic phytoplankton have a small global biomass but play major roles in primary production and climate. Despite improved understanding of phytoplankton diversity and evolution, we largel...

In-cell quantitative structural imaging of phytoplankton using 3D electron microscopy

Archive ouverte | Uwizeye, Clarisse | CCSD

Phytoplankton is a minor fraction of the global biomass playing a major role in primary production and climate. Despite improved understanding of phytoplankton diversity and genomics, we lack nanoscale subcellular imaging approach...

Chargement des enrichissements...