A Prophage in Diabetic Foot Ulcer–Colonizing Staphylococcus aureus Impairs Invasiveness by Limiting Intracellular Growth

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Rasigade, Jean-Philippe | Dunyach-Remy, Catherine | Sapin, Anaïs | Messad, Nourredine | Trouillet-Assant, Sophie | Dupieux, Céline | Lavigne, Jean-Philippe | Laurent, Frédéric

Edité par CCSD ; Oxford University Press -

International audience. The mechanisms that drive the transition from commensality to invasiveness in Staphylococcus aureus are poorly understood. We recently reported that >50% of S. aureus isolates from uninfected diabetic foot ulcers in French patients harbor a prophage, ROSA-like, that is absent from invasive isolates from diabetic foot infections, including osteomyelitis. Here we show that the ROSA-like insertion abolishes the ability of S. aureus to replicate within osteoblasts, the bone-forming cells, greatly reducing damage to infected cells. These results unravel an important mechanism by which particular S. aureus strains are maintained in a commensal state in diabetic foot ulcers.

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