Estimation of HIV-1 DNA Level Interfering with Reliability of HIV-1 RNA Quantification Performed on Dried Blood Spots Collected from Successfully Treated Patients

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Zida, Sylvie | Tuaillon, Edouard | Barro, Makoura | Kwimatouo Lekpa Franchard, Arnaud | Kagoné, Thérèse | Nacro, Boubacar | Ouedraogo, Abdoul Salam | Bolloré, Karine | Sanosyan, Armen | Plantier, Jean-Christophe | Meda, Nicolas | Sangare, Lassana | Rouzioux, Christine | Rouet, François | Kania, Dramane

Edité par CCSD ; American Society for Microbiology -

International audience. The impact of HIV-1 DNA coamplification during HIV-1 RNA quantification on dried blood spots (DBS) was explored. False-positive HIV RNA detection (22/62, 35%) was associated with high HIV-1 DNA levels. Specificity of HIV-1 RNA assays on DBS should be evaluated following manufacturer protocols on samples with HIV-1 DNA levels of ≥1,000 copies/10(6) peripheral blood mononuclear cells.

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