What to expect from molecular tools for non-documented pediatric infectious diseases

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Filleron, Anne | Michon, Anne-Laure | Jumas-Bilak, Estelle | Jeziorski, Eric | Zorgniotti, Isabelle | Tran, Tu Anh | Filleron, Thomas | Rodière, Michel | Marchandin, Hélène

Edité par CCSD ; Expert Reviews (formerly Future Drugs) -

International audience. Objective: Evaluation of the contribution of molecular tools to the overall diagnosis of infectious diseases in children.Methods: Results of 16S rDNA analysis (179 children; 228 specimens), combined to specific amplification of Kingella kingae (126 children; 166 osteoarticular specimens), were retrospectively analyzed for samples with inconclusive cultures.Result: The overall positive yield in diagnosis was 12.8% of the patients for 16S rDNA PCR, 40.5% for K. kingae PCR and 45.2% for combined use of both methods. Results were related to clinical and biological data (direct examination, certainty/uncertainty of clinical diagnosis, fever, biological markers, previous antibiotics), and to the number of samples analyzed per patient, allowing the identification of specific situations with significant contribution of PCR methods.Conclusion: Molecular techniques constitute valuable tools to improve the bacterial infection diagnosis in children; however, specific indications, dedicated samples, and number of analyzed samples per patient are key points to optimize their contribution.

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