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Variability and conservation of structural domains in divide-and-conquer approaches

Archive ouverte | Wiegand, Thomas | CCSD

International audience. The use of protein building blocks for the structure determination of multidomain proteins and protein–protein complexes, also known as the “divide and conquer” approach, is an important stra...

Solid-state NMR chemical-shift perturbations indicate domain reorientation of the DnaG primase in the primosome of Helicobacter pylori.

Archive ouverte | Gardiennet, Carole | CCSD

International audience. We here investigate the interactions between the DnaB helicase and the C-terminal domain of the corresponding DnaG primase of Helicobacter pylori using solid-state NMR. The difficult crystall...

Monitoring ssDNA binding to the DnaB helicase from $Helicobacter\ pylori$ by solid-state NMR spectroscopy

Archive ouverte | Wiegand, Thomas | CCSD

International audience. DnaB helicases are bacterial, ATP-driven enzymes that unwind double-stranded DNA during DNA replication. Herein, we study the sequential binding of the "non-hydrolysable" ATP analogue AMP-PNP...

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