0 avis
Environmental factors shaping cultured free-living amoebae and their associated bacterial community within drinking water network
Archive ouverte
Edité par CCSD ; IWA Publishing/Elsevier -
International audience. Free-living amoebae (FLA) constitute an important part of eukaryotic populations colonising drinkingwater networks. However, little is known about the factors influencing their ecology in such environments.Because of their status as reservoir of potentially pathogenic bacteria, understanding environmentalfactors impacting FLA populations and their associated bacterial community is crucial. Throughsampling of a large drinking water network, the diversity of cultivable FLA and their bacterial communitywere investigated by an amplicon sequencing approach, and their correlation with physicochemicalparameters was studied. While FLA ubiquitously colonised the water network all year long, significantchanges in population composition were observed. These changes were partially explained by severalenvironmental parameters, namely water origin, temperature, pH and chlorine concentration. Thecharacterisation of FLA associated bacterial community reflected a diverse but rather stable consortiumcomposed of nearly 1400 OTUs. The definition of a core community highlighted the predominance ofonly few genera, majorly dominated by Pseudomonas and Stenotrophomonas. Co-occurrence analysis alsoshowed significant patterns of FLA-bacteria association, and allowed uncovering potentially new FLA -bacteria interactions. From our knowledge, this study is the first that combines a large sampling schemewith high-throughput identification of FLA together with associated bacteria, along with their influencingenvironmental parameters. Our results demonstrate the importance of physicochemical parametersin the ecology of FLA and their bacterial community in water networks.
Consulter en ligne
Suggestions
Du même auteur
