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Artificial binding proteins (Affitins) as probes for conformational changes in secretin PulD.

Archive ouverte | Krehenbrink, M. | CCSD

International audience. The DNA-binding protein Sac7d was previously modified to bind with high affinity to the N domain of the outer membrane secretin PulD from the bacterium Klebsiella oxytoca. Here, we show that ...

A Single Amino Acid Substitution Changes the Self-Assembly Status of a Type IV Piliation Secretin

Archive ouverte | Nickerson, N. N. | CCSD

International audience. Secretins form large multimeric complexes in the outer membranes of many Gram-negative bacteria, where they function as dedicated gateways that allow proteins to access the extracellular envi...

Three-dimensional structure by cryo-electron microscopy of YvcC, an homodimeric ATP-binding cassette transporter from Bacillus subtilis.

Archive ouverte | Chami, M. | CCSD

International audience. YvcC, a multidrug transporter from Bacillus subtilis, is a member of the ATP-binding cassette superfamily, highly homologous to each half of human multidrug-resistance P-glycoprotein and to s...

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