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Genetic Instability and Associated Genome Plasticity in Streptomyces ambofaciens: Pulsed-Field Gel Electrophoresis Evidence for Large DNA Alterations in a Limited Genomic Region

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Leblond, Pierre | Demuyter, Philippe | Simonet, Jean-Marc | Decaris, Bernard

Edité par CCSD ; American Society for Microbiology -

International audience. Using pulsed-field gel electrophoresis (PFGE) analysis, the amplifiable units of DNA (AUD) loci AUD6 andAUD90 of Streptomyces ambofaciens DSM40697 could be mapped in the wild-type genome within two adjacentAseI restriction fragments estimated to be about 75 and 850 kb. In addition, the genetic instability andformation of very large deletions were strictly correlated. Their sizes were estimated to range from 250 to morethan 2,000 kb. These deletions affected the DNA region overlapping both amplifiable loci. PFGE also allowedus to localize the amplified DNA sequences and to establish their structure: amplification takes place at the AUDlocus as a tandem array of the wild-type AUD sequence.

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