Syracuse

Cellular morphometric analysis: from microscopic scale to whole mouse brains

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You, Zhenzhen | Vandenberghe, Michel | Balbastre, Yael | Souedet, Nicolas | Herard, Anne-Sophie | Delzescaux, Thierry

Edité par CCSD ; Springer -

International audience. In neurodegenerative diseases, pathological aggregates disturb cell function and morphology. Quantifying these changes is of prime interest but raises experimental and computational challenges. In this context, whole-slide imaging (WSI) offers the unique opportunity to analyze whole mouse brain sections at the cellular level using a variety of histological markers. However, this technique generates terabytes of data which is difficult to fully analyze.We developed a novel method enabling: (1) to detect cells and pathological aggregates in WSI color images at the cellular level; (2) to quantify parameters of interest such as density, shape, location or color and (3) to integrate the information within quantitative and multi-scale heat maps.This original approach enables to extract pertinent information from high-resolution qualitative images and to dramatically reduce the amount of information to be processed. A supplementary step of this work consists in extending the analysis from brain sections to the entire brains reconstructed in 3D using our in-house software BrainVISA (http://brainvisa.info).From the generated 3D parametric maps, voxel-wise statistical studies can be performed to investigate cellular structural alterations without a priori. Furthermore, correlating 3D whole-brain parametric maps with in vivo imaging modalities (MRI, fMRI, PET, in vivo microscopy, etc.) will improve the understanding of the relationship between brain structure and function in disease.

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