Evaluation of a new extraction protocol for yeast identification by mass spectrometry

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Gouriet, Frédérique | Ghiab, Farida | Couderc, Carine | Bittar, Fadi | Tissot Dupont, Hervé | Flaudrops, Christophe | Casalta, Jean-Paul | Sambe-Ba, Bissoume | Fall, Bécaye | Raoult, Didier | Fenollar, Florence

Edité par CCSD ; Elsevier -

International audience. In this paper, we evaluate a rapid and safe pretreatment procedure using glass beads for MALDI-TOF yeast identification in a routine clinical laboratory avoiding the use of formic acid. We created a new yeast database library using 1186 yeasts, including 11 references strains. The database was tested using 2131 clinical isolates allowing accurate species-level identification in 98.9% (2107/2131) of cases with a score over 1.9 and in 99% (2123/2131) of the strains at the genus level. The new protocol is a rapid, reliable and safe procedure for the accurate identification of pathogenic Candida strains and requires minimal handling.

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