Afficher la couverture 'Estimating the population abundance of tissue-infiltrating immune and stromal cell populations using gene expression | Becht, Etienne' en grand format
/5

0 avis

Estimating the population abundance of tissue-infiltrating immune and stromal cell populations using gene expression

Archive ouverte

Becht, Etienne | Giraldo, Nicolas A. | Lacroix, Laetitia | Buttard, Bénédicte | Elarouci, Nabila | Petitprez, Florent | Selves, Janick | Laurent-Puig, Pierre | Sautès-Fridman, Catherine | Fridman, Wolf H. | de Reyniès, Aurélien, A

Edité par CCSD ; BioMed Central -

International audience. We introduce the Microenvironment Cell Populations-counter (MCP-counter) method, which allows the robust quantification of the absolute abundance of eight immune and two stromal cell populations in heterogeneous tissues from transcriptomic data. We present in vitro mRNA mixture and ex vivo immunohistochemical data that quantitatively support the validity of our method's estimates. Additionally, we demonstrate that MCP-counter overcomes several limitations or weaknesses of previously proposed computational approaches. MCP-counter is applied to draw a global picture of immune infiltrates across human healthy tissues and non-hematopoietic human tumors and recapitulates microenvironment-based patient stratifications associated with overall survival in lung adenocarcinoma and colorectal and breast cancer.

Suggestions

Du même auteur

Tumor cells hijack macrophage-produced complement C1q to promote tumor growth

Archive ouverte | Roumenina, Lubka | CCSD

International audience. Clear-cell renal cell carcinoma (ccRCC) possesses an unmet medical need, particularly at the metastatic stage, when surgery is ineffective. Complement is a key factor in tissue inflammation, ...

Quantitative Analyses of the Tumor Microenvironment Composition and Orientation in the Era of Precision Medicine

Archive ouverte | Petitprez, Florent | CCSD

International audience. Tumors are formed by aggregates of cells of various origins including malignant, stromal and immune cells. The number of therapies targeting the microenvironment is increasing as the tumor mi...

Tertiary lymphoid structures generate and propagate anti-tumor antibody-producing plasma cells in renal cell cancer

Archive ouverte | Meylan, Maxime | CCSD

International audience. The presence of intratumoral tertiary lymphoid structures (TLS) is associated with positive clinical outcomes and responses to immunotherapy in cancer. Here, we used spatial transcriptomics t...

Chargement des enrichissements...