Functional metagenomics: construction and high-throughput screening of fosmid libraries for discovery of novel carbohydrate-active enzymes

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Ufarté, Lisa | Bozonnet, Sophie | Laville, Elisabeth | Cecchini, Davide | Serin, Sandra | Jacquiod, Samuel | Demanèche, Sandrine | Simonet, Pascal | Franqueville, Laure | Veronese, Gabrielle

Edité par CCSD ; Springer -

Activity-based metagenomics is one of the most efficient approaches to boost the discovery of novel biocatalysts from the huge reservoir of uncultivated bacteria. In this chapter, we describe a highly generic procedure of metagenomic library construction and high-throughput screening for carbohydrate-active enzymes. Applicable to any bacterial ecosystem, it enables the swift identification of functional enzymes that are highly efficient, alone or acting in synergy, to break down polysaccharides and oligosaccharides.

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