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Rapid bacterial identification, resistance, virulence and type profiling using selected reaction monitoring mass spectrometry

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Charretier, Yannick | Dauwalder, Olivier | Franceschi, Christine | Degout-Charmette, Elodie | Zambardi, Gilles | Cecchini, Tiphaine | Bardet, Chloé | Lacoux, Xavier | Dufour, Philippe | Veron, Laurent | Rostaing, Hervé | Lanet, Véronique | Fortin, Tanguy | Beaulieu, Corinne | Perrot, Nadine | Dechaume, Dominique | Pons, Sylvie | Girard, Victoria | Salvador, Arnaud | Durand, Géraldine | Mallard, Frédéric | Theretz, Alain | Broyer, Patrick | Chatellier, Sonia | Gervasi, Gaspard | van Nuenen, Marc | Roitsch, Carolyn Ann | van Belkum, Alex | Lemoine, Jérôme | Vandenesch, François | Charrier, Jean-Philippe

Edité par CCSD ; Nature Publishing Group -

International audience. Mass spectrometry (MS) in Selected Reaction Monitoring (SRM) mode is proposed for in-depth characterisation of microorganisms in a multiplexed analysis. Within 60-80 minutes, the SRM method performs microbial identification (I), antibiotic-resistance detection (R), virulence assessment (V) and it provides epidemiological typing information (T). This SRM application is illustrated by the analysis of the human pathogen Staphylococcus aureus, demonstrating its promise for rapid characterisation of bacteria from positive blood cultures of sepsis patients.

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