A Member of a New Picornaviridae Genus Is Shed in Pig Feces

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Sauvage, Virginie | Ar Gouilh, Meriadeg | Cheval, Justine | Muth, Erika | Pariente, Kévin | Burguiere, Ana | Caro, Valérie | Manuguerra, Jean-Claude | Eloit, Marc

Edité par CCSD ; American Society for Microbiology -

During a study of the fecal microbiomes from two healthy piglets using high-throughput sequencing (HTS), we identified a viralgenome containing an open reading frame encoding a predicted polyprotein of 2,133 amino acids. This novel viral genome displayedthe typical organization of picornaviruses, containing three structural proteins (VP0, VP3, and VP1), followed by sevennonstructural proteins (2A, 2B, 2C, 3A, 3B, 3Cpro , and 3Dpol). Given its particular relationship with Parechovirus, we propose toname it “Pasivirus” for Parecho sister clade virus, with “Swine pasivirus 1” (SPaV1) as the type species. Fecal samples collected atan industrial farm from healthy sows and piglets from the same herd (25 and 75, respectively) with ages ranging from 4 to 28weeks were analyzed for the presence of SPaV1 by one-step reverse transcription (RT)-PCR targeting a 3D region of 151 bp.SPaV1 was detected in fecal samples from 51/75 healthy piglets (68% of the animals) and in none of the 25 fecal samples fromhealthy sows, indicating that SPaV1 circulates through enteric infection of healthy piglets. We propose that SPaV1 represents thefirst member of a novel Picornaviridae genus related to parechoviruses.

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