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In situ determination of collagen fibrils size via absolute measurements of second harmonic generation signals

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Bancelin, Stéphane | Aimé, Carole | Gusachenko, Ivan | Kowalczuk, Laura | Latour, Gael | Coradin, Thibaud | Schanne-Klein, Marie-Claire

Edité par CCSD ; SPIE -

International audience. We correlated SHG and Electron Microscopies to calibrate SHG signals as a function of collagen fibril diameter, down to 30 nm. We observed a fourth power dependence, in agreement with analytical and numerical calculations. We applied this calibration to abnormal fibrils in the Descemet’s membrane of intact unfixed rat corneas.

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