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Determinants for membrane association of the hepatitis C virus NS2 protease domain.

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Lange, Christian M | Bellecave, Pantxika | Dao Thi, Viet Loan | Tran, Huong T L | Penin, François | Moradpour, Darius | Gouttenoire, Jérôme

Edité par CCSD ; American Society for Microbiology -

Affiliations ECOFECT. International audience. Hepatitis C virus (HCV) nonstructural protein 2 (NS2) is required for HCV polyprotein processing and particle assembly. It comprises an N-terminal membrane domain and a C-terminal, cytosolically oriented protease domain. Here, we demonstrate that the NS2 protease domain itself associates with cellular membranes. A single charged residue in the second α-helix of the NS2 protease domain is required for proper membrane association, NS2 protein stability, and efficient HCV polyprotein processing.

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