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Comparative localization and functional activity of the main hepatobiliary transporters in HepaRG cells and primary human hepatocytes.
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Edité par CCSD ; Oxford University Press (OUP) -
International audience. The role of hepatobiliary transporters in drug-induced liver injury remains poorly understood. Various in vivo and in vitro biological approaches are currently used for studying hepatic transporters; however, appropriate localization and functional activity of these transporters are essential for normal biliary flow and drug transport. Human hepatocytes (HH) are considered as the most suitable in vitro cell model but erratic availability and inter-donor functional variations limit their use. In the present work, we aimed to compare localization of influx and efflux transporters and their functional activity in differentiated human HepaRG hepatocytes with fresh HH in conventional (CCHH) and sandwich (SCHH) cultures. All tested influx and efflux transporters were correctly localized to canalicular (BSEP, MRP2, MDR1, MDR3) or basolateral (NTCP, MRP3) membrane domains and were functional in all models. Contrary to other transporters, NTCP and BSEP were less abundant and active in HepaRG cells, cellular uptake of taurocholate was 2.2- and 1.4-fold and bile excretion index 2.8-and 2.6- fold lower, than in SCHH and CCHH respectively. However, when taurocholate canalicular efflux was evaluated in standard and divalent cation-free conditions in buffers or cell lysates, the difference between the three models did not exceed 9.3%. Interestingly, cell imaging showed higher bile canaliculi contraction/relaxation activity in HepaRG hepatocytes and larger bile canaliculi networks in SCHH. Altogether, our results bring new insights in mechanisms involved in bile acids accumulation and excretion in HH and suggest that HepaRG cells represent a suitable model for studying hepatobiliary transporters and drug-induced cholestasis.
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