Pluripotency : in embryo and [i][/i]in vitro[i][/i]

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Schmaltz-Panneau, Barbara | Jouneau, Luc | Daniel, Nathalie | Osteil, Pierre | Tapponnier, Yann | Afanassieff, Marielle | Savatier, Pierre | Dube, Delphine | Veillard, Anne-Clémence | Jouneau, Alice | Beaujean, Nathalie | Duranthon, Véronique

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Session 9: Epigenomics
Session 9: Epigenomics. During embryonic development, two distinct cell populations appear at the blastocyststage: the differentiated trophectoderm lineage and the inner cell mass (ICM) whose pluripotent cells are able to colonize all the lineages of the fetus. Later on, the still pluripotent epiblast and the hypoblast segregate from the ICM. In the mouse, two kinds of pluripotent stem cells can be derived in vitro from the embryo : naïve pluripotent ESC cells (obtained from the ICM) and primed pluripotent EpiSC cells (derived from the epiblast). Our analyses evidence a role for DNA methylation in the epigenetic barrier between these two pluripotent states. More recently, induced pluripotent stem cells (iPS) have been obtained by reprogramming somatic cells in vitro. In most domestic animal species, research are still necessary to obtain naïve pluripotent stem cells from embryo and true iPS from differentiated cells. To characterize pluripotent cells in the rabbit, we first analyzed the transcriptome of the "in embryo" pluripotent cells compared to that of their differentiated counterparts. Then, in an attempt to assess the "quality" of rabbit "ES" and iPS cells, we characterized their transcriptome fingerprint and compared it to that of rabbit ICM and epiblasts. Our data evidence major differences between rabbit "in embryo" and "in vitro" derived pluripotent cells. These differences will be discussed in regards to naïve and primed pluripotency

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