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Development of an Efficient In Vivo System (P-junc-TpaseIS(1223)) for Random Transposon Mutagenesis of Lactobacillus casei

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Licandro-Seraut, Helene, H. | Brinster, Sophie, S. | van de Guchte, Maarten | Scornec, Helene, H. | Maguin, Emmanuelle | Sansonetti, Philippe | Cavin, Jean-François | Serror, Pascale

Edité par CCSD ; American Society for Microbiology -

The random transposon mutagenesis system P-junc-TpaseIS(1223) is composed of plasmids pVI129, expressing IS1223 transposase, and pVI110, a suicide transposon plasmid carrying the P-junc sequence, the substrate of the IS1223 transposase. This system is particularly efficient in Lactobacillus casei, as more than 10,000 stable, random mutants were routinely obtained via electroporation.

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