Afficher la couverture 'The [i]Arabidopsis[/i] nitrate transporter NRT2.4 plays a double role in roots and shoots of nitrogen-starved plants | Kiba, Takatoshi, T.' en grand format
/5

0 avis

The [i]Arabidopsis[/i] nitrate transporter NRT2.4 plays a double role in roots and shoots of nitrogen-starved plants

Archive ouverte

Kiba, Takatoshi, T. | Feria-Bourrellier, Ana-Belen, A.B. | Lafouge, Florence, F. | Lezhneva, Lina, L. | Boutet-Mercey, Stephanie, S. | Orsel, Mathilde, M. | Bréhaut, Virginie, V. | Miller, Anthony, A. | Daniel-Vedele, Francoise, Vedele | Sakakibara, Hitoshi, H. | Krapp, Annemarie, A.

Edité par CCSD ; American Society of Plant Biologists (ASPB) -

L'article original est publié par The American Society of Plant Biologists. Plants have evolved a variety of mechanisms to adapt to N starvation. NITRATE TRANSPORTER2.4 (NRT2.4) is one of seven NRT2 family genes in Arabidopsis thaliana, and NRT2.4 expression is induced under N starvation. Green fluorescent protein and beta-glucuronidase reporter analyses revealed that NRT2.4 is a plasma membrane transporter expressed in the epidermis of lateral roots and in or close to the shoot phloem. The spatiotemporal expression pattern of NRT2.4 in roots is complementary with that of the major high-affinity nitrate transporter NTR2.1. Functional analysis in Xenopus laevis oocytes and in planta showed that NRT2.4 is a nitrate transporter functioning in the high-affinity range. In N-starved nrt2.4 mutants, nitrate uptake under low external supply and nitrate content in shoot phloem exudates was decreased. In the absence of NRT2.1 and NRT2.2, loss of function of NRT2.4 (triple mutants) has an impact on biomass production under low nitrate supply. Together, our results demonstrate that NRT2.4 is a nitrate transporter that has a role in both roots and shoots under N starvation.

Suggestions

Du même auteur

Natural variation for carbohydrate content in Arabidopsis. Interaction with complex traits dissected by quantitative genetics

Archive ouverte | Calenge, Fanny | CCSD

International audience. Besides being a metabolic fuel, carbohydrates play important roles in plant growth and development, in stress responses, and as signal molecules. We exploited natural variation in Arabidopsis...

Arabidopsis roots and shoots show distinct temporal adaptation patterns toward nitrogen starvation

Archive ouverte | Krapp, Annemarie, A. | CCSD

Nitrogen (N) is an essential macronutrient for plants. N levels in soil vary widely, and plants have developed strategies to cope with N deficiency. However, the regulation of these adaptive responses and the coordinating signals ...

Disruption of the nitrate transporter genes AtNRT2.1 and AtNRT2.2 restricts growth at low external nitrate concentration

Archive ouverte | Orsel, Mathilde, Orsel Baldwin | CCSD

International audience. The high-affinity transport systems in Arabidopsis thaliana (L.) Heynh. involve potentially seven genes. Among these, the AtNRT2.1 and/or AtNRT2.2 genes have been shown to play a major role i...

Chargement des enrichissements...