Towards construction of an ultra high density linkage map for Pinus pinaster

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Ritter, Enrique | Aragonés, Ana | Markussen, Torsten | Acheré, Virginie | Espinel, Santiago | Fladung, Matthias | Wrobel, Sandra | Faivre-Rampant, Patricia | Jeandroz, Sylvain | Favre, Jean-Michel

Edité par CCSD ; Springer Nature (since 2011)/EDP Science (until 2010) -

International audience. Two parental linkage maps have been constructed from the P. pinaster reference population (0024 $\times$ C803) based on AFLP, SSR and EST markers. Although segregating polymorphism was low due to a high degree of homozygosity in the parents, 12 linkage groups with 26 to 46 markers each were obtained for each parent. The availibility of 70 anchor points based on fragments common to both parents and based on codominant SSR and EST markers allowed to determine homologous chromosomes for both maps and to construct one integrated map. Total genome length of the integrated map is around 2000 cM including 1182 markers. Since some of the EST and SSR markers are also mapped in different pine species, association of linkage groups of our reference population with those of other published maps was possible. . Vers la construction d'une carte génétique ultra-haute densité chez Pinus pinaster. Deux cartes génétiques ont été construites à partir d'un croisement intra-spécifique de P. Pinaster (0024 Landes $\times$ C803 Corse) avec des marqueurs AFLP, SSR et EST. Malgré un faible polymorphisme dû à la forte homozygotie des parents, 12 groupes de liaison comprenant 26 à 46 marqueurs ont été obtenus pour chacune des cartes. La présence de 70 points d'ancrage déterminés à partir des fragments communs aux deux parents a permis d'identifier les chromosomes homologues des deux cartes et de construire une carte consensus d'une longueur totale d'environ 2000 cM et comprenant 1182 marqueurs. La présence de quelques marqueurs EST et microsatellites déjà cartographiés chez différentes espèces de pins a permis d'aligner un certain nombre de groupes de liaison avec cette carte de pin maritime.

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