Syracuse

Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution.

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Armache, Jean-Paul | Jarasch, Alexander | Anger, Andreas M | Villa, Elizabeth | Becker, Thomas, S. | Bhushan, Shashi | Jossinet, Fabrice | Habeck, Michael | Dindar, Gülcin | Franckenberg, Sibylle | Marquez, Viter | Mielke, Thorsten | Thomm, Michael | Berninghausen, Otto | Beatrix, Birgitta | Söding, Johannes | Westhof, Eric | Wilson, Daniel N | Beckmann, Roland

Edité par CCSD ; National Academy of Sciences -

International audience. Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Triticum aestivum) 80S ribosome at 5.5-Å resolution. This map, together with a 6.1-Å map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model ∼98% of the rRNA. Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES-ES and r-protein-ES interactions, providing insight into the structure and evolution of the eukaryotic ribosome.

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