Complete circular DNA in the mitochondria-like organelles of Blastocystis hominis.

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Wawrzyniak, Ivan | Roussel, Michaël | Diogon, Marie | Couloux, Arnaud | Texier, Catherine | Tan, Kevin S W | Vivarès, Christian | Delbac, Frédéric | Wincker, Patrick | El Alaoui, Hicham

Edité par CCSD ; Elsevier -

International audience. Blastocystis hominis is an anaerobic parasite of the human intestinal tract belonging to the Stramenopile group. Using genome sequencing project data, we describe here the complete sequence of a 29,270-bp circular DNA molecule that presents mitochondrial features (such as oxidative phosphorylation complex I subunits) but lacks complexes III, IV and V. Transmission electron microscopy analyses reveal that this molecule, as well as mitochondrial (NADH:ubiquinone oxidoreductase subunit 7 (NAD7), beta-succinyl-CoA synthetase (beta-SCS)) and hydrogenosomal (pyruvate ferredoxin oxido-reductase (PFOR), iron-hydrogenase) proteins, are located within double-membrane surrounded-compartments known as mitochondria-like organelles (MLOs). As there is no evidence for hydrogen production by this organism, we suggest that MLOs are more likely anaerobic mitochondria.

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