Hypersensitivity pneumonitis and metalworking fluids contaminated by Mycobacteria.

Archive ouverte

Tillie-Leblond, I. | Grenouillet, F. | Reboux, G. | Roussel, S. | Chouraki, B. | Lorthois, C. | Dalphin, J.-C. | Wallaert, B. | Millon, L.

Edité par CCSD ; European Respiratory Society -

International audience. Metalworking fluids are responsible for hypersensitivity pneumonitis (MWF-HP). The aim is to identify the antigen (Ag) responsible for MWF-HP and to optimize serological diagnosis by definition of a threshold allowing discrimination between HP patients from asymptomatic exposed workers (H-MWF) Thirteen patients who were workers at a car-engine manufacturing plant, were suspected of MWF-HP. Microbial analysis of 83 used MWF was carried out. Sera from 13 MWF-HP, 12 H-MWF and 18 UC (healthy unexposed controls) were tested to determine their immunological responses to three Ag including Mycobacterium immunogenum (Mi). Mi was identified in 40% of used fluids by culture and confirmed by DNA sequence. The threshold to differentiate MWF-HP from H-MWF was five arcs of precipitation (Sensitivity (Se) 77%, specificity (Sp) 92%) as determined by electrosyneresis. Using ELISA methods with protein extract from Mi, a threshold leading to (Se) 92% and (Sp) 100%) was established. The detection of specific antibodies against Mi Ag in high level in case sera, suggests that Mi contaminated-MWF is responsible for MWF-HP. To discriminate MWF-HP from H-MWF, we also put a five arcs threshold forward by electrosyneresis and a 1.6 arbitrary unit for ELISA methods threshold.

• Description
• Sujet/Public
• Outil
• Outil d'anticipation
• Mémoire et thèse
• Gestion