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D-amino acid detection in peptides by MALDI-TOF-TOF.

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Sachon, Emmanuelle | Clodic, Gilles | Galanth, Cécile | Amiche, Mohamed | Ollivaux, Céline | Soyez, Daniel | Bolbach, Gérard

Edité par CCSD ; American Chemical Society -

International audience. Detection of a D-amino acid residue in natural peptides by mass spectrometry remains a challenging task, as this post-translational modification does not induce any change in molecular mass. To our knowledge, the present article is the first report using matrix-assisted laser desorption/ionization (MALDI) for the discrimination and the quantification of peptide isomers. In this work, we used synthetic hepta- and decapeptides of biological relevance and their isomers. All-L sequences and some isomers containing a D-residue in various positions were analyzed.

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