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Brain natriuretic peptide secretion in adult rat heart muscle cells: The role of calcium channels

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Nader, Laurence | Smayra, Viviane | Jebara, Victor | Bois, Patrick | Potreau, Daniel | Fares, Nassim

Edité par CCSD ; Elsevier ; Société française de cardiologie [2008-....] -

International audience. The cellular mechanisms that regulate B-type natriuretic secretion are not well elicited. Intracellular fluctuation of calcium ions rate seems to be implicated. AIMS: In this study, we evaluate the role of ventricular transmembrane calcium channels in the secretion of BNP in normal adult rats (group N) and pressure overload hypertrophied ones (group H). METHODS: We measured plasma BNP concentration and BNP concentration in culture media of cardiomyocytes from N and H group in the presence and absence of calcium channels antagonists. RESULTS: Plasma BNP concentration was increased in H group in comparison to N group (0.630+/-0.008 ng/ml versus 0.106+/-0.004 ng/ml; p<0.01). This increase in BNP level was also obtained in culture media of H group in comparison to N group (3.45+/-0.7 ng/ml versus 0.53+/-0.22 ng/ml). However, the presence of calcium channels antagonists in the culture media of cardiomyocytes had decreased BNP concentration in both N (nifedipine: 0.22+/-0.04 ng/ml; verapamil: 0.19+/-0.05 ng/ml; diltiazem: 0.17+/-0.03 ng/ml; p<0.05) and H group (nifedipine: 0.18+/-0.05 ng/ml; verapamil: 0.23+/-0.04 ng/ml; diltiazem: 0.28+/-0.1 ng/ml; p<0.05). CONCLUSION: These results suggest that transmembrane calcium channels may have an important role in the regulation of BNP secretion.

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