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High-yield production of secreted active proteins by the Pseudomonas aeruginosa type III secretion system.

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Derouazi, M. | Toussaint, Bertrand | Quénée, Lauriane | Epaulard, Olivier | Guillaume, Mathilde | Marlu, Raphaël | Polack, Benoît

Edité par CCSD ; American Society for Microbiology -

International audience. The Escherichia coli system is the system of choice for recombinant protein production because it is possible to obtain a high protein yield in inexpensive media. The accumulation of protein in an insoluble form in inclusion bodies remains a major disadvantage. Use of the Pseudomonas aeruginosa type III secretion system can avoid this problem, allowing the production of soluble secreted proteins.

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