Improvement of FISH mapping resolution on combed DNA molecules by iterative constrained deconvolution: a quantitative study.

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Monier, K. | Heliot, L. | Rougeulle, C. | Heard, E. | Robert-Nicoud, M. | Vourc'H, C. | Bensimon, A. | Usson, Yves

Edité par CCSD ; S Karger AG -

Image restoration approaches, such as digital deconvolution, are becoming widely used for improving the quality of microscopic images. However, no quantification of the gain in resolution of fluorescence images is available. We show that, after iterative constrained deconvolution, fluorescent cosmid signals appear to be 25% smaller, and 1.2-kb fragment signals on combed molecules faithfully display the expected length.

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