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MtHAP2-1 is a key transcriptional regulator of symbiotic nodule development regulated by microRNA169 in Medicago truncatula.

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Combier, Jean-Philippe | Frugier, Florian | de Billy, Françoise | Boualem, Adnane | El-Yahyaoui, Fikri | Moreau, Sandra | Vernié, Tatiana | Ott, Thomas | Gamas, Pascal | Crespi, Martin | Niebel, Andreas

Edité par CCSD ; Cold Spring Harbor Laboratory Press -

In the model legume Medicago truncatula, we identified a new transcription factor of the CCAAT-binding family, MtHAP2-1, for which RNA interference (RNAi) and in situ hybridization experiments indicate a key role during nodule development, possibly by controlling nodule meristem function. We could also show that MtHAP2-1 is regulated by microRNA169, whose overexpression leads to the same nodule developmental block as MtHAP2-1 RNAi constructs. The complementary expression pattern of miR169 and MtHAP2-1 and the phenotype of miR169-resistant MtHAP2-1 nodules strongly suggest, in addition, that the miR169-mediated restriction of MtHAP2-1 expression to the nodule meristematic zone is essential for the differentiation of nodule cells.

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