Afficher la couverture 'A glucose biosensor based on modified-enzyme incorporated within electropolymerised poly(3,4-ethylenedioxythiophene) (PEDT) films | Piro, Benoît' en grand format
/5

0 avis

A glucose biosensor based on modified-enzyme incorporated within electropolymerised poly(3,4-ethylenedioxythiophene) (PEDT) films

Archive ouverte

Piro, Benoît | Lan Anh, Dang | Minh Chau, Pham | Fabiano, Silvia | Canh, Tran-Minh

Edité par CCSD ; Elsevier -

International audience. We have constructed and characterised a glucose sensor using glucose oxidase (GOD) covalently attached to carboxylic acid polyethyleneglycol (PEG), called (PEG–GOD). This modified enzyme was entrapped afterwards within poly(3,4-ethylenedioxythiophene) (PEDT) films electrogenerated on glassy carbon (GC) electrodes. The composite (PEG–GOD/PEDT) film is more porous than the film without enzyme (PEDT+PEG). Data from electrochemical quartz microbalance (ECQM) and pH-stat experiments indicate a good relative activity of the modified enzyme, ca. 12–15%. Amperometric measurements, using ferrocenemethanol as the redox mediator, confirms that the modified enzyme is catalytically active. The effect of film thickness was also investigated. The sensitivities were quite similar for modified-GOD electrodes (ca. 3 mA cm−2 M−1) and unmodified-GOD electrodes (ca. 2.7 mA cm−2 M−1) but a better stability was obtained with modified PEG–GOD electrodes.

Suggestions

Du même auteur

Poly 3,4-ethylenedioxythiophene as an entrapment support for amperometric enzyme sensor

Archive ouverte | Fabiano, Silvia | CCSD

International audience. A conducting polymer of poly 3,4-ethylenedioxythiophene (PEDT) was used as a matrix for entrapment of enzymes onto a platinum electrode surface in order to construct amperometric biosensors. ...

The use of enzymes for oxygenator membranes

Archive ouverte | Broun, Georges | CCSD

Two applications of immobilized enzymes to the artificial oxygenation of blood are described. The first concerns facilitated transport of CO2 moving from the blood through a hydrophobic membrane. It is based on activation of the r...

Electrodes potentiométriques pour le dosage des gaz en phase gazeuse

Archive ouverte | Canh, Tran-Minh | CCSD

International audience. Des électrodes potentiométriques sensibles aux gaz sont réalisées en vue de doser les gaz en phase gazeuse. On donne le principe de fonctionnement de ces électrodes, les conditions nécessaire...

Chargement des enrichissements...