Afficher la couverture 'A Proximity Ligation-Based Method for Quantitative Measurement of D-Loop Extension in S. cerevisiae | Piazza, Aurele' en grand format
/5

0 avis

A Proximity Ligation-Based Method for Quantitative Measurement of D-Loop Extension in S. cerevisiae

Archive ouverte

Piazza, Aurele | Koszul, Romain | Heyer, Wolf-Dietrich

Edité par CCSD -

Methods in enzymology (0076-6879). International audience. Homologous recombination faithfully restores the sequence information interrupted by a DNA double-strand break by referencing an intact DNA molecule as a template for repair DNA synthesis. DNA synthesis is primed from 3'-OH end of the invading DNA strand in the displacement loop (D-loop). Here, we describe a simple and quantitative proximity ligation-based assay to study the initiation of homologous recombination-associated DNA synthesis initiated at the D-loop and final product formation. The D-loop extension assay overcomes the semiquantitative nature and some limitations of the current PCR-based technique and facilitates the study of the recombination-associated DNA synthesis.

Suggestions

Du même auteur

Dynamic Processing of Displacement Loops during Recombinational DNA Repair

Archive ouverte | Piazza, Aurele | CCSD

International audience. Displacement loops (D-loops) are pivotal intermediates of homologous recombination (HR), a universal DNA double strand break (DSB) repair pathway. We developed a versatile assay for the physi...

Characterizing meiotic chromosomes' structure and pairing using a designer sequence optimized for Hi‐C

Archive ouverte | Muller, Héloise | CCSD

International audience. In chromosome conformation capture experiments (Hi-C), the accuracy with which contacts are detected varies due to the uneven distribution of restriction sites along genomes. In addition, rep...

Role of G-quadruplexes in the induction of genomic instability in S. cerevisiae. Rôle des G-quadruplexes dans l'instabilité génomique chez Saccharomyces cerevisiae

Archive ouverte | Piazza, Aurele | CCSD

G-quadruplexes are topologically diverse four-stranded structures formed by certain G-rich nucleic acids in physiological conditions in vitro. During my thesis, I studied their in vivo formation, and the pathological consequences ...

Chargement des enrichissements...