Genetic analysis of Thailand hantavirus in Bandicota indica trapped in Thailand

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Hugot, Jean-Pierre | Plyusnina, Angelina | Herbreteau, Vincent | Nemirov, Kirill | Laakkonen, Juha | Lundkvist, Ake | Suputtamongkol, Yupin | Henttonen, Heikki | Plyusnin, Alexander

Edité par CCSD ; BioMed Central -

Sixty one tissue samples from several rodent species trapped in five provinces of Thailand were examined for the presence of hantaviral markers by enzyme-immunoassay and immunoblotting. Four samples, all from the great bandicoot rat Bandicota indica, were confirmed positive for the hantaviral N-antigen. Two of them were trapped in Nakhon Pathom province, the other two in Nakhon Ratchasima province, approximately 250 km from the other trapping site. When analysed by RT-nested PCR, all four rodents were found positive for the hantaviral S- and M-segment nucleotide sequences. Genetic analysis revealed that the four newly described wild-type strains belong to Thailand hantavirus. On the phylogenetic trees they formed a well-supported cluster within the group of Murinae-associated hantaviruses and shared a recent common ancestor with Seoul virus.

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