New Transcriptional Reporters to Quantify and Monitor PPAR γ Activity

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Degrelle, Severine, S. | Shoaito, Hussein | Fournier, Thierry

Edité par CCSD ; Hindawi Publishing Corporation -

International audience. The peroxisome-proliferator-activated-receptor-(PPAR) is a member of the nuclear receptor superfamily that plays a critical role in diverse biological processes, including adipogenesis, lipid metabolism, and placental development. To study the activity of PPAR , we constructed two new reporter genes: a fluorescent GFP-tagged histone-2B (PPRE-H2B-eGFP) and a secreted nanoluciferase (PPRE-pNL1.3[secNluc]). This study demonstrates their usage to monitor PPAR activity in different cell types and screen for PPAR 's potential ligands.

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