Afficher la couverture 'Comparison of assays measuring extracellular vesicle tissue factor in plasma samples: communication from the ISTH SSC Subcommittee on Vascular Biology | Bonifay, Amandine' en grand format
/5

0 avis

Comparison of assays measuring extracellular vesicle tissue factor in plasma samples: communication from the ISTH SSC Subcommittee on Vascular Biology

Archive ouverte

Bonifay, Amandine | Mackman, Nigel | Hisada, Yohei | Sachetto, Ana Teresa Azevedo | Hau, Chi | Gray, Elaine | Hogwood, John | Aharon, Anat | Badimon, Lina | Barile, Lucio | Baudar, Justine | Beckmann, Lennart | Benedikter, Birke | Bolis, Sara | Bouriche, Tarik | Brambilla, Marta | Burrello, Jacopo | Camera, Marina | Campello, Elena | Ettelaie, Camille | Faille, Dorothée | Featherby, Sophie | Franco, Corentin | Guldenpfennig, Maite | Hansen, John-Bjarne | Judicone, Coralie | Kim, Yohan | Kristensen, Soren Risom | Laakmann, Katrin | Langer, Florian | Latysheva, Nadezhda | Lucien, Fabrice | de Menezes, Erika Marques | Mullier, François | Norris, Philip | Nybo, Jette | Orbe, Josune | Osterud, Bjarne | Paramo, Jose | Radu, Claudia | Roncal, Carmen | Samadi, Nazanin | Snir, Omri | Suades, Rosa | Wahlund, Casper | Chareyre, Corinne | Abdili, Evelyne | Martinod, Kimberly | Thaler, Johannes | Dignat-George, Françoise | Nieuwland, Rienk | Lacroix, Romaric

Edité par CCSD ; Wiley -

International audience. BackgroundScientific and clinical interest in extracellular vesicles (EVs) is growing. EVs that expose tissue factor (TF) bind factor VII/VIIa and can trigger coagulation. Highly procoagulant TF-exposing EVs are detectable in the circulation in various diseases, such as sepsis, COVID-19, or cancer. Many in-house and commercially available assays have been developed to measure EV-TF activity and antigen, but only a few studies have compared some of these assays.ObjectivesThe International Society on Thrombosis and Haemostasis Scientific and Standardization Committee Subcommittee on Vascular Biology initiated a multicenter study to compare the sensitivity, specificity, and reproducibility of these assays.MethodsPlatelet-depleted plasma samples were prepared from blood of healthy donors. The plasma samples were spiked either with EVs from human milk or EVs from TF-positive and TF-negative cell lines. Plasma was also prepared from whole human blood with or without lipopolysaccharide stimulation. Twenty-one laboratories measured EV-TF activity and antigen in the prepared samples using their own assays representing 18 functional and 9 antigenic assays.ResultsThere was a large variability in the absolute values for the different EV-TF activity and antigen assays. Activity assays had higher specificity and sensitivity compared with antigen assays. In addition, there was a large intra-assay and interassay variability. Functional assays that used a blocking anti-TF antibody or immunocapture were the most specific and sensitive. Activity assays that used immunocapture had a lower coefficient of variation compared with assays that isolated EVs by high-speed centrifugation.ConclusionBased on this multicenter study, we recommend measuring EV-TF using a functional assay in the presence of an anti-TF antibody.

Suggestions

Du même auteur

Increasing the sensitivity of the human microvesicle tissue factor activity assay

Archive ouverte | Vallier, Loris | CCSD

International audience. Introduction: The TF-FVIIa complex is the primary activator of coagulation. Elevated levels of microvesicle (MV) bearing tissue factor (TF)-dependent procoagulant activity are detectable in p...

Methods for the identification and characterization of extracellular vesicles in cardiovascular studiesfrom exosomes to microvesicles

Archive ouverte | Davidson, Sean | CCSD

International audience. Extracellular vesicles (EVs) are nanosized vesicles with a lipid bilayer that are released from cells of the cardiovascular system, and are considered important mediators of intercellular and...

Granulocyte microvesicles with a high plasmin generation capacity promote clot lysis and improve outcome in septic shock

Archive ouverte | Cointe, Sylvie | CCSD

International audience. Abstract Microvesicles (MVs) have previously been shown to exert profibrinolytic capacity, which is increased in patients with septic shock (SS) with a favorable outcome. We, therefore, hypot...

Chargement des enrichissements...