0 avis
Deciphering the bacterial interaction network within seafood microbiome to develop a sustainable biopreservation strategy
Archive ouverte
Edité par CCSD -
International audience. Seafood products and their storage conditions are suitable for the development of microorganisms originating from fish and seawater or resulting from contamination during the food chain processing. They are thus highly susceptible to spoilage due to biochemical and microbial degradations and are considered as potential vectors of human pathogens (e.g Listeria monocytogenes). Biopreservation is a microbiome engineering strategy based on bacterial competition. It consists in fighting against undesirable bacteria by inoculating protective microorganisms or their metabolites exhibiting antimicrobial activities in food. Despite the undeniable inhibition effect of selected lactic acid bacteria (e.g Carnobacterium spp.) against Listeria monocytogenes, the associated sensory impact of the biopreservative strain addition and its effect on spoilers varies according to the composition of the product microbiota. This microbiota is closely linked to the processing and the storage conditions.To understand the effect of the addition of protective culture on all bacterial species of microbiome, omics strategies were combined to establish the bacterial interaction network and assess the competitions related to the production of antimicrobial compounds. One hundred strains representative of salmon microbiome diversity were selected from the laboratory collection. Miniaturized cross inhibition between all strains, representing a total of 10 000 assays, were performed. Inhibition profiles of strains were then related to the presence of antimicrobial biosynthetic clusters, thanks to sequencing, assembly and annotation of all genomes. The results provided an exhaustive description of the bacterial interaction network and antimicrobial diversity. In fact, between 0 and 23 antimicrobial biosynthetic clusters could be identified per genome. Half of them encoded peptide molecules like unmodified bacteriocins, ribosomally synthesized and post-translationally modified peptides (RiPPs) and non ribosomal peptides (NRPs). Then, culturomic and transcriptomic approaches were combined to determine the abiotic and biotic factors that impact the synthesis of active peptide during salmon storage. Three bioprotective Carnobacterium strains harbouring different bacteriocin equipments were firstly investigated.
Consulter en ligne
Suggestions
Du même auteur
