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Natural Recombination Event within the Capsid Genomic Region Leading to a Chimeric Strain of Human Enterovirus B

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Bouslama, Lamjed | Nasri, Dorsaf | Chollet, Lionel | Belguith, Khaoula | Bourlet, Thomas | Aouni, Mahjoub | Pozzetto, Bruno | Pillet, Sylvie

Edité par CCSD ; American Society for Microbiology -

International audience. ABSTRACT Recombination between two strains is a known phenomenon for enteroviruses replicating within a single cell. We describe a recombinant strain recovered from human stools, typed as coxsackievirus B4 (CV-B4) and CV-B3 after partial sequencing of the VP1 and VP2 coding regions, respectively. The strain was neutralized by a polyclonal CV-B3-specific antiserum but not by a CV-B4-specific antiserum. The nucleotide sequence analysis of the whole structural genomic region showed the occurrence of a recombination event at position 1950 within the VP3 capsid gene, in a region coding for the 2b antigenic site previously described for CV-B3. This observation evidences for the first time the occurrence of an interserotypic recombination within the VP2-VP3-VP1 capsid region between two nonpoliovirus enterovirus strains. The neutralization pattern suggests that the major antigenic site is located within the VP2 protein.

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