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A simplified model to study the role of LysM-RLKs during mycorrhization
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Edité par CCSD -
International audience. Land plants can intracellularly accommodate soil microorganisms to obtain nutrients, thus forming ecologically important endosymbiosis. Most land plants form a symbiosis with arbuscular mycorrhizal fungi (AMS) to acquire water and phosphorus, while nitrogen-fixing symbiosis with rhizobia (NFS) is limited to legumes (Oldroyd, 2013). Genetic studies on NFS have revealed the crucial roles of plasma membrane-anchored LysM Receptor-Like Kinases in perceiving microbial-derived molecules that are essential for establishing NFS (Buendia et al., 2018). In contrast, loss-of-function mutants of LysM-RLKs in angiosperms only have quantitative effects on AMS establishment, raising the question of whether AMS formation strictly requires the host's perception by LysM-RLKs of diffusible fungal-derived molecules, similar to its NFS counterpart. Given that the expanded LysM-RLKs families in model angiosperms may lead to functional redundancy, we conducted a systematic study using the liverwort Marchantia paleacea, which has only four LysM-RLKs in its genome. We generated single CRISPR/Cas9 loss-of-function mutants and examined their ability to host AMS. Interestingly, mutants of the CERK1 orthologue (MpaLYKa) completely lost the ability to establish AMS. On the other hand, mutants of the single LYR gene retained their ability to form AMS, as did mutants of both MpaLYKb and MpaLYKc. To investigate potential interactions among LysM-RLKs, we are currently generating higher order mutants, including the single LysM Receptor-Like-Protein MpaLYM. Studying similar mutants in a Marchantia background expressing the calcium reporter aequorin will enable us to assess their function in response to microbial compounds such as chitin oligomers and/or lipochitooligosaccharidic signals. Overall, our findings suggest that Marchantia paleacea is a suitable model for studying receptor-driven signaling events during AMS.
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